Coding

Part:BBa_K1975002

Designed by: Joachim Steen Larsen   Group: iGEM16_UNIK_Copenhagen   (2016-06-28)


PhaA - Beta-kethothiolase

This is a Beta-Ketoehiolase (commonly known as PhaA). This enzyme catalyzes the first step in the production of the bioplastic PHB. The reaction it catalyzes is two molecules of acetyl-CoA is being putting together to one molecule of acetoacetyl-CoA under the usage of a propanoate that will be converted to acetate. By combining it with the synthetic gene, LPE it can be used in the synthesis of P(LA-co-3HB).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None